تفاصيل الوثيقة
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نوع الوثيقة |
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مقال في مجلة دورية |
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عنوان الوثيقة |
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A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis |
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لغة الوثيقة |
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الانجليزية |
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المستخلص |
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Amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms (SNPs) and insertion/deletions (indels) that currently are assayable using various instrumental analysis methods including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. This paper describes an approach to establish objective analytical thresholds suitable for multiplexed PCR-MPS methods. A definition is proposed for PCR-MPS method background noise, and an analytical threshold based on background noise is described. |
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ردمد |
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1932-6203 |
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اسم الدورية |
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PLOS ONE |
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المجلد |
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12 |
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العدد |
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5 |
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سنة النشر |
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1438 هـ
2017 م |
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نوع المقالة |
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مقالة علمية |
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تاريخ الاضافة على الموقع |
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Wednesday, June 26, 2019 |
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الباحثون
| Brian Young | Young, Brian | باحث | دكتوراه | brian@nichevision.com |
| Jonathan L. King | King, Jonathan L. | باحث | دكتوراه | |
| Bruce Budowle | Budowle, Bruce | باحث | دكتوراه | |
| Luigi Armogida | Armogida, Luigi | باحث | دكتوراه | |
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الرجوع إلى صفحة الأبحاث
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